Year: 2018 | Month: December | Volume 8 | Issue 6
Isolation and Characterization of the Newcastle Disease Virus (NDV) of Haryana Region Based on F-gene Sequence
Abstract:
Newcastle disease virus (NDV) continues to cause serious economic losses in global chicken production. The objective of the present study was to use a rapid and efficient method for determining pathotype of NDV of poultry from field specimens by using reverse transcription polymerase chain reaction (RT-PCR) and in silico restriction endonuclease enzymes (REA) method. The field samples were investigated for identification, differentiation and pathotyping of NDV by using amplification of RT-PCR followed by in silico REA of F protein encoding gene. The RT-PCR amplification for F gene NDV generated DNA fragments in size of 356 bp. To differentiate virulent and avirulent NDV, the RT-PCR product of F gene in size of 356 base pairs (bp) was analyzed by HhaI and BglI restriction enzymes. Out of one hundred twenty field samples examined, nineteen were found positive for NDV and among these nineteen positive samples, sixteen samples were lentogenic, two samples were mesogenic and one sample was velogenic NDV. RT-PCR combined with in silico REA method by using HhaI and BglI enzymes can be used to determine pathotype of NDV from field specimens.
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